An Electrospun Mat Composed of Collagen and Polycaprolactone Loaded with Low Concentration of Silver Sulfadiazine Can Promote Wound Healing in Rats

Designing a nanofibrous composite with a suitable thickness using natural and synthetic materials containing antibiotic agents with controlled release can be an appealing and critical research topic. A synthetic polymer like polycaprolactone (PCL) and a natural polymer such as collagen (COLL) possess considerable potentials in the domain of tissue regeneration. In this study, an electrospun blend of PCL and COLL type-I was loaded with low concentration of silver sulfadiazine (SSD) as an antimicrobial agent and then this characterized mat was applied in vivo.PCL/COLL membrane containing 0.1% SSD with 500 μm thickness was prepared via electrospinning. Viscosity of solutions, characterization of nanofibers, presence of SSD in nanofibers, surface hydrophilicity of nanofibers, release pattern of SSD, antimicrobial properties, as well as cellular viability using human dermal fibroblasts (HDFs) on the scaffold were also examined. A model of rat wound was then applied to assess the effects of PCL/COLL/SSD scaffold on wound healing process in vivo.This combination showed appropriate normal characteristics with desirable skin wound healing. The low concentration of SSD also revealed in vitro antibacterial effects against Staphylococcus aureus (S.aureus) and Pseudomonas aeruginosa (P.aeruginosa). Moreover, attachment and spreading of HDFs on this nanofibrous composite was reported to be in an optimal condition. As well, in vivo evaluation demonstrated that full repaired skin had been observed on day 21 in the PCL/COLL/SSD group after being wounded.The PCL/COLL/SSD mat could be considered as a suitable wound dressing of nanofibrous membranes in future in order to shorten healing time and also prevent scarring and infection during wound healing process.

کلیدواژه ها:

Silver Sulfadiazine ، Antibacterial Wound Dressing ، Wound Healing ، Drug Release ، Polycaprolactone/Collagen Electrospinning