Identification of relationship between athero-miRs with the ribosome biogenesis pathway in recurrent acute coronary syndrome by transcriptome analysis

Introduction & Objectives: Atherosclerosis is a chronic inflammatory disease that begins with the accumulation of fat-filled foam cells in the intimal layer of the artery. MiR-712 is known as a murine mechanosensitive miRNA regulated by disturbed flow in endothelial cells derived from an unexpected source, the primary ribosomal RNA, and targeting TIMP3 pro-atherogenic reactions. In this study, a human athero-miR with a seed region similar to this microRNA was screened for targetome and mapped to the transcriptome of patients PBMCs with recurrent acute coronary syndrome.Materials & Methods: A set of genes with 3'UTR potential to bind to the seed region of interest was obtained from TargetScanHuman7.2 databases and analyzed for pathway enrichment in KEGG repository data. Also, blood cell transcriptome of patients with recurrent acute coronary syndrome was extracted from GSE34822 dataset with downregulation by p-value <0.01 and this gene set was also analyzed for pathway enrichment in KEGG repository data. Taken, and compared with the results of the previous step. The miRNA-mRNAs interaction network was analyzed and visualized with Cytoscape3.6 software.Results: Blood transcriptome analysis of patients with recurrent acute coronary syndrome revealed that the ribosome biogenesis pathway was suppressed and genes including EBNA1BP2 and NSA2 were potential targets of athero-miRs in this pathway.Conclusion: Interestingly, some other studies confirm the role of the ribosome and ribosomal L13a protein in macrophages in susceptibility to atherosclerosis in mice. Therefore, according to the results of our study, targeting mechanosensitive athero-miRs associated with ribosome biogenesis may suggest a new therapeutic pattern in atherosclerosis recurrence.

کلیدواژه ها:

Coronary heart disease recurrence ، athero-miR ، ribosome biogenesis ، transcriptome analysis