Bioinformatics evaluation of hsa-miR-542-3p signaling pathways and its associated rs3088012 function in patients with chronic myeloid leukemia

Introduction & Objectives: Chronic Myeloid Leukemia (CML) is a slow-growing cancer of the blood-forming tissue. In chronic myeloid leukemia the bone marrow produces too many white blood cells. CML is characterized by the presence of the Philadelphia chromosome (Ph+), which contains the oncogenic BCR-ABL1 fusion gene resulting from a translocation between chromosomes 9 and 22. ABL1 is mutated in 8.82% of CML patients.Materials & Methods: To get more information we used NCBI, miRNASNP, miRBase, miRWALK2.0, DAVID databases and KEGG pathway as the bioinformatics tools.Results: Investigations at the rs3088012 locus indicated that T allele altered to C, which may effect on function of the miRNA associated with this region. Finally, hsa-miR-542-3p as one of the most important miRNAs related to ABL1 and given the confirmation of the role of hsa-miR-542-3p in CML through bioinformatics databases, this miRNA selected for this analysis.Conclusion: The studies due to rs3088012 have shown that when the SNP locus is in the mutant C allele, the miRNA binds to it less strongly than the wild T allele. So when this SNP occurs, hsa-miR-542-3p has less inhibitory effect on it and ABl1 is transcribed more. Due to the inhibitory action of MDM2 on p53, we predicted that a person with this harmful polymorphism is more susceptible to CML by increasing the expression of the ABL1.

کلیدواژه ها:

Chronic Myeloid leukemia ، hsa-miR-542-3p ، CML ، ABL1 ، KEGG pathway