Bioinformatics evaluation of hsa-miR-1289 signaling pathways and its associated rs10277413 function in patients with Non-small-cell lung cancer

Introduction & Objectives: Non-small-cell lung cancers (NSCLCs) are the most common lung cancers and account for more than 80% of all lung cancers. Overexpression of EGFR has been reported and implicated in the pathogenesis of many human malignancies, including NSCLC.Materials & Methods: To get more information we used NCBI, miRNASNP, miRBase, miRWALK2.0, DAVID databases and KEGG pathway as the bioinformatics tools.Results: Investigations at the rs10277413 locus indicated that T allele altered to A and G, which may effect on function of the miRNA associated with this region. Finally, hsa-miR-1289 as one of the most important miRNAs related to EGFR and given the confirmation of the role of hsa-miR-1289 in NSCLC through bioinformatics databases, this miRNA selected for this analysis.Conclusion: The studies due to rs10277413 have shown that when the SNP locus is in the mutant A or G allele, the miRNA binds to it less strongly than the wild T allele. So when this SNP occurs, hsa-miR-1289 has less inhibitory effect on it and EGFR is transcribed more. Due to role EGFR in cell proliferation, we predicted that a person with this harmful polymorphism is more susceptible to NSCLC by increasing the expression of the EGFR.

کلیدواژه ها:

Non-small-cell lung cancer ، hsa-miR-1289 ، NSCLC ، EGFR ، KEGG pathway