Metabolic Aspects of Irisin in human health and biomedical regenerative medicine

In my laboratory, we have pinpointed functional aspects of Fndc5 which was recognized as a kind of myokine and could be cleaved and secrets as a hormone, termed Irisin with an important role in thermogenesis and energy homeostasis. Within one decade our colleagues were involved in unraveling the function and expression of FNDC5 in a number of cell types. We have chased the expression of Fndc5 during the neural differentiation of both mouse and human embryonic stem cells. On the other hand, we analyzed the different splicing variant form of Fndc5. Using gain/loss of function approach we indicated that Fndc5may play a prerequisite step in neural differentiation of embryonic stem cells as well as cardiac differentiation. Furthermore, we did an intensive search on the structure Fndc5 promoter. As the expression of Fndc5 up-regulated post retinoic acid treatment during neural differentiation of mouse embryonic stem cells. This interesting data motivated ass to check whether this enhancement is observable in neural differentiation of human embryonic stem cells. Therefore, results showed that all Fndc5 mRNA isoforms have a similar increasing pattern. Also, we explored that RA enhanced Fndc5 expression through a non-genomic pathway through the ERK signaling pathway. Interestingly, we identified that Fndc5 acts in PPARγ/PGC1-Fndc5 axis in cardiac differentiation of embryonic stem cells.

کلیدواژه ها:

Cardiac differentiation ، Embryonic stem cells ، Fndc5 ، Neural differentiation