Prevalence of racR gene in thermophilic Campylobacter jejuni and Campylobacter coli isolated in poultry

Although, many genes are involved in pathogenesis of Campylobacter, racR gene considered the main gene for Campylobacter pathogenicity in humans.The purpose of this study was determined the prevalence of recR gene in Campylobacter jejuni and Campylobacter coli isolated from poultry. To perform the study 196 fecal samples were collected from poultry. All samples were subjected for isolation of Campylobacter spp. using prêt KB method and the presumptive isolates authenticated by DNA sequencing of 16srRNA genes. Finally, Campylobacter isolates assessed for detection of racR gene. The results obtained indicated that 39 strains of Campylobacter spp. were isolated. Of all isolates 30 strains were identified as Campylobacter jejuni and the rest (9 isolates) was Campylobacter coli. The genome of 24 (80%) C.jejuni and 9 (100%) C.coli werecontained racR gene. Hence based on foregoing evidence racR gene existed approximately in all isolates. Therefore, Campylobacter strains isolated from different sources could be considered infectious agent for human. It is because phenotypical character inducted by racR gene (colonization and heat tolerance) helps them to survive and cause infection.

کلیدواژه ها:

racR gene ، Thermophilic Campylobacters ، Poultry