Tissue engineering has emerged as the knowledge of designing and fabricating new tissue to recover the function of damaged organs or defunct tissues, relying on three key components of stem cells, growth factors, and scaffolds to overcome the limitations of tissue transplantation. In tissue engineering, the design of 3D scaffolds with appropriate physical and mechanical properties and the use of effective composites for faster tissue regeneration is important. One of the most effective ways to increase the mechanical properties of the scaffold is to use ceramic as a reinforcement phase. Reduced graphene oxide (rGO) is one of the bioactive ceramics that, in combination with the scaffold, improves the mechanical and biological properties of the nanocomposite and has made it as an effective factor in tissue engineering. Therefore, in this study, the application of rGO in bone, heart and nerve tissue engineering has been investigated.Methods. This study was a systematic review study in which validated articles in both human and animal domains regarding the use of graphene nanomaterials and its derivatives in bone, nerve and myocardium, via search banks and Latin databases, such as: PubMed, Latin ProQuest, google scholar, and Elsevier And Persian such as: MedLib, Magiran, Noormags, Iran Medex, SID, National library and archives of I.R. Iran that some keyword such as Graphene, Graphene Oxide(GO), rGO, myocardium, bone scaffold and nerve were searched from 2010 to 2019 and finally 25 articles were studied.Findings:The combination of rGO in bone tissue engineering scaffolds can improve their physical, chemical and mechanical properties. In addition, functional scaffolds withrGO can induce stem cell differentiation to osteoblasts as well as improve the biomineralization process, that has able them the ability of bone therapy. Because,Graphene-based nanomaterials have excellent electrical and mechanical properties and attractive surface chemistry, they are good candidates for cardiac tissue engineering.Its reported, due to high electrical conductivity and the unique morphology of the wrinkled chemical surface of rGO, it can promote the growth and differentiation of neural stem cells that mimics the matrix surrounding the nerve cells. Discussion. it can be concluded that among graphene derivatives, rGO has significant potential in tissue engineering due to its unique chemical structure, attractive mechanical and electrical properties.