In Vitro Evaluation of the Effects of Zearalenone and α-Zearalenol on MCF-7 and MDA-MB-468 Cell Lines of Human Breast Cancer
محل انتشار: مجله بین المللی پزشکی رضوی، دوره: 3، شماره: 4
سال انتشار: 1394
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 584
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شناسه ملی سند علمی:
JR_RIJO-3-4_004
تاریخ نمایه سازی: 6 اسفند 1398
چکیده مقاله:
Background: In the field of human breast cancer, the most recent researches referred to the influence of endogenous estrogen or exposure to environmental estrogen, as risk factors. Zearalenone (ZEN) as a mycotoxin and its derivative α- zearalenol (α-ZOL) are known nonsteroidal estrogenic compounds with potential endocrine disrupting properties.Objectives: The present study was designed to investigate the effects of ZEN and α-ZOL on human breast cancer cell lines MCF-7 and MDA-MB-468.Materials and Methods: Cell lines were treated by low and high doses of ZEN and α-ZOL (0, 1, 30, 62, 125, 250 and 500 ng/ml and 1, 2, 4, 8, 15, 30, 62 and 125 μg/ml) for 24 and 48 hours. Then MTT colorimetric assay was used to evaluate the cytotoxicity effect of ZEN and α-ZOL. Furthermore, morphological changes of treated and untreated cell lines were studied under an inverted microscope.Results: The results obtained from the present study demonstrated that both ZEN and α-ZOL enhance the cell viability of MCF-7 especially at low doses (1 - 500 ng/ml) and at a high dose of 125μg/ml after 24 and 48 hours. However, this effect for α-ZOL was somewhat greater than that for ZEN. On the other hand, these estrogenic compounds did not have any effect on the cell viability of MDA-MB-468. No morphological change was observed in treated cells.Conclusions: These results show that ZEN and α-ZOL enhance the rate of cell division in ER positive cells and therefore, exposure to this mycotoxin may increase the risk of breast cancer.
کلیدواژه ها:
نویسندگان
Rahmatollah Parandin
Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, IR Iran
Morteza Behnam Rassouli
Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, IR Iran
Sajjad Sisakhtnezhad
Department of Biology, Faculty of Sciences, Razi University of Kermanshah, Kermanshah, IR Iran
Nasser Mahdavi Shahri
Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, IR Iran