Survey of Polymerase Chain Reaction Efficiency in the Detection of Mycoplasma, Listeria and Brucella in Culture Negative Samples Obtained from Women with Abortion

Introduction and Objectives: Misabortion is a disorder resultant of numerous causes such as infections. It is clear that bacterial infections due to genital mycoplasma, Listeria and brucella can cause septic abortion. A right time diagnosis of these infections can improve women fertility. This study was conducted to survey the efficiency of PCR in detecting the specimens with negative culture results in women suffering from septic abortion. We also did this by considering individual variables. Material & Methods: In this analytical and descriptive study, appropriate samples were collected from a total of 87 women with septic abortion who referred to Karaj’s hospitals. The samples were cultured into mycoplasma, Listeria and brucella specific media. Then, the bacterial genus was verified by different biochemical tests. PCR test is performed on negative culture cases and SPSS-18 software is used for the statistical analysis of data. Results: From the total of 87 blood samples, 37 samples (42.5 %) were positive for mycoplasma and ureaplasma (25 cases) and Listeria monosytogenes (12 cases) with both culture and PCR method. Our result showed no positive cases for brucella. From the total cultured specimens, 12 cases (13.8%) were positive and 75 cases (86.2%) were negative. We performed PCR test for negative cultured results. With PCR method, 25 cases (33.33 %) showed positive results and 50 cases (66.67%) showed negative results. The results also showed a significant statistical relation between PCR test results with recurrent abortion and level of education (P< 0.05). Conclusion: The results show that PCR is a more sensitive, easier and faster method in comparison to culture for detecting bacterial cause septic abortion. It is obvious that quick diagnostic and starting antimicrobial therapy at the right time can prevent and decrease abortion’s complications, so it recommended then that using PCR in detecting this bacterial cause septic abortion can be more effective.

کلیدواژه ها:

Septic Abortion ، Culture ، PCR