Diagnosis of Mycoplasma Pneumonia in Patients with Respiratory Tract Infections Using Three Methods of Culture, ELISA and PCR Molecular in Tehran Province, Iran

سال انتشار: 1398
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 414

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شناسه ملی سند علمی:

MEDISM20_233

تاریخ نمایه سازی: 26 بهمن 1398

چکیده مقاله:

Introduction and objectives: Mycoplasma pneumonia is the most commonly Mycroplasma species cause of human pathogens, including upper respiratory tract infection and atypical pulmonary pneumonia. Mycoplasma pneumoniae is also known to be one of the most commonly pathogens causing community-acquired pneumonia (CAP) in some populations, especially in children. Materials and Methods: In this study, 100 samples of patients with respiratory infections (52% female and 48% male, mean age of 53/62 years and range from 17 to 85 years old) were collected. Selection of suspected cases of Mycoplasma pneumoniae respiratory tract infections was conducted with the diagnosis of a super pulmonologist based on clinical symptoms. For the ELISA test, serum samples, PCR and culture in PPLOBroth liquid medium and PPLOagar solid medium from throat swab samples of patients with respiratory tract infections were used. In this study, Mycoplasma pneumonia standard strain (ATCC: 29342) was used. Results: In this study, 17 samples (17%) for Mycoplasma (16SrRNA gene) and 6 (6%) for existence of Mycoplasma pneumonia (P1 gene) were reported positive, using specific primers. 37 cases (37%) were positive for ELISA and 14 (14%) colonies related to mycoplasma in PPLOAgar medium. Conclusion: The results of this study showed that Mycoplasma pneumonia is one of the pathogens that cause respiratory tract infections in humans. The PCR molecular method is a quick and sensitive technique which has a high sensitivity and specificity in comparison with other methods such as ELISA and culture.

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نویسندگان

Iman Pouladi

Department of Medical Microbiology, Faculty of Medicine, Shahed University of Medical Sciences, Tehran, Iran.

Reza Mirnejad

Molecular Biology Research Center, System biology and Poisoning institute, Baqiyatallah University of Medical Sciences, Tehran, Iran

Ghasem Azimi

Departement of Internal diseases, Shahid mostafa khomeni Hospital, Tehran, Iran

Mohammad Niakan

Department of Medical Microbiology, Faculty of Medicine, Shahed University of Medical Sciences, Tehran, Iran.

Mohsen Taheri

Department of Medical Microbiology, Faculty of Medicine, Shahed University of Medical Sciences, Tehran, Iran.