Assessment of the In vitro antifungal efficacy of soil Streptomycetes isolates against Fusarium oxysporum and Fusarium solani
محل انتشار: بیستمین کنگره بین المللی میکروب شناسی ایران
سال انتشار: 1398
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 447
نسخه کامل این مقاله ارائه نشده است و در دسترس نمی باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
MEDISM20_166
تاریخ نمایه سازی: 26 بهمن 1398
چکیده مقاله:
Introduction and Objectives: Members of the genus Fusarium are common soil saprophytes and significant plant pathogens, which also cause a wide spectrum of diseases ranging from superficial to the life-threatening systemic infections in human as well as animals. Since the Streptomycetes are the largest taxon of antibiotic producers we aimed to assess the inhibitory effects of Streptomyces isolated from soil samples against Fusarium species. Materials and Methods: Soil samples were collected from different regions of Kerman city in order to isolation of Streptomyces. Morphological as well as physiological characterization of the isolates was investigated according to the standard protocols. All isolates were evaluated in order to their antifungal activities against Fusarium oxysporum (PTCC 5115), and Fusarium solani (PTCC 5284). Afterwards, molecular identification of active Streptomyces isolates was conducted using 16Sr DNA gene. Result: Out of 250 soil collected samples, fifty Streptomyces isolates were obtained. Among these isolates two strains showed the most antagonistic in vitro effect on F. oxysporum and F. solani. Besides, these two Streptomyces isolates were showed valuable lipase, amylase, protease, and Chitinase activities. According to the analysis of 16S rRNA gene sequences these isolates were identified as Streptomyces rochei (99% similarity). Conclusion: The obtained results of this study indicated that S. rochei has obvious inhibitory effect against Fusarium species which could use as a potent source of bioactive compounds with antifungal activity.
کلیدواژه ها:
نویسندگان
Azam Amanizadeh
Department of Medical Parasitology and Mycology University of Medical Sciences, Kerman, IR Iran.
Seyyed Amin Ayatollahi Mousavi
Department of Medical Mycology and Parasitology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, IR Iran.
Gholam Hossein Shahidi Bonjar
Department of Plant Pathology, Faculty of Agricultural Engineering, Shahid Bahonar University of Kerman, Kerman, IR Iran.