Detection of bla-IMP, bla-VIM and bla-spm genes among Pseudomonas aeruginosaisolated from clinical isolates in Tehran, Iran

Introduction and Objectives: Carbapenems are considered first-line drugs for the treatment of multi-drug resistant bacteria, including Pseudomonas aeruginosa. Unfortunately, carbapenem-resistant P. aeruginosa (CRPA) has been increasing in recent years. The aim of this study was to determine antibiotic susceptibility patterns and metallo‐beta‐lactamase (MBL)-mediated resistance in clinical P. aeruginosa isolates. Materials and methods: In this cross-sectional study, 270 Pseudomonas aeruginosa isolates were collected from the various clinical specimens in Tehran hospitals from 2014 to 2016. After identification of isolates by biochemical tests then antibiotic susceptibility and detection of MBLs using the Kirby‐Bauer and combined double‐disk synergy test methods, respectively. The frequency of bla-IMP and bla-VIM and bla –SPM among MBL producing P. aeruginosa was investigated using Polymerase Chain Reaction (PCR). Results: In this study, cephpodoxim and polimixin with 98.6% and 1.7% resistance showed the highest and lowest resistance against isolates. Moreover, 165 (61.1%) of isolates were detected as multi drug resistance strains. A total of 270 isolates, 112 (41.4%) isolates were carbapenem- resistant. From 112 isolates, 51 (45.5%) isolates were metallo-beta-lactamase producer which 48 isolates (42.8%) and 33 isolates (29.4%) carried blaIMP-1 and blaVIM-1 genes, respectively. Also 12 (10.7%) isolates carried both of the blaIMP and blaVIM genes simultaneously. Conclusion: The prevalence of MBLs-producing Pseudomonas aeruginosa strains detected in this study is a main concern and highlights the need for infection control measures are needed to prevent further dissemination of these organisms.

کلیدواژه ها:

Carbapenem ، Metallo‐β‐lactamase genes ، Pseudomonas aeruginosa ، PCR ، MDR