Evaluation of DNA damage in a Her2+ cell line induced by an Auger-emitting immunoconjugate

سال انتشار: 1395
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 354

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شناسه ملی سند علمی:

JR_IRJNM-24-2_005

تاریخ نمایه سازی: 21 بهمن 1398

چکیده مقاله:

Introduction: Auger electron based radioimmunotherapy (RIT) using 111In-DOTA-trastuzumab (111In-DOTA-antiHer2) feasibility was studied in vitro on a HER2/neu positive cell line, SkBr3. Methods:111In-DOTA-antiHer2 was prepared according to the optimized conditions followed by quality control tests including radiochemical purity; immunoreactivity). SkBr3 as a HER2/neu positive cell line was used to determine the degree of internalization and cell viability of 111In-DOTA-antiHer2. For comet assay freshly cultivated SkBr3 cells incubated with 111In-DOTA-antiHer2 in 3.7 and 7.4 MBq doses at 37ºC for 24h. Results:111In-DOTA-antiHer2 (> 95% radiochemical purity; > 79% immunoreactivity) demonstrated significant internalization in SKBr3 cells in 24 h. no significant cell viability difference observed for 111In-DOTA-antiHer2 and 111In cation treatments. Comet assay at 3.7 MBq demonstrated no significant DNA damage, while at 7.4 MBq dose DNA damage observed at least 16% more than control In-111 chloride after 24 h. Conclusion: Although the internalization of 111In-DOTA-antiHer2 was approved in this study, however, lack of cell death and slight DNA breakage for 111In-DOTA-antiHer2 treatment suggests absence of nucleus entry which is essential for demonstration of DNA damage according to Auger electron range at cellular level.

نویسندگان

Behnaz Piroozfar

Radiation Application Research School, Nuclear Science and Technology Research Institute, Tehran, Iran

Behrouz Alirezapoor

Radiation Application Research School, Nuclear Science and Technology Research Institute, Tehran, Iran

Farahnaz Motamedi Sedeh

Radiation Application Research School, Nuclear Science and Technology Research Institute, Tehran, Iran

Amir Reza Jalilian

Radiation Application Research School, Nuclear Science and Technology Research Institute, Tehran, Iran

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