Evaluation of Memory Enhancement Effects of 4-(4-trifluromethylphenyl)-2-Aminopyrimidine Using Behavioral Tests in Mice

Background and Objective: There are several models for memory impairment in mice, one of which is centered on the anticholinergic drug scopolamine. Due to its antagonistic properties on muscarinic receptors, scopolamine impairs memory functions -especially memory acquisition and consolidation- and leads to anterograde amnesia. The novel aminopyrimidine compound, (4-(4-trifluromethylphenyl)-2-aminopyrimidine), suspected to ameliorate memory impairment is tested using this model as a basis for investigation.Materials and Methods: a total of 32 mice were divided evenly and randomly into 4 groups of 8 signifying different concentrations of the test compounds with 16 additional mice divided evenly acting as the negative control group (NaCl only) and positive control group (scopolamine only). On the first day, mice were injected intraperitoneally with scopolamine (3mg/kg) and 30 minutes later with the test compound (40, 20, 10, or 5mg/kg). 30 minutes after test compound injection, the passive avoidance test was performed according to standard protocol. On the second day, there were no injections and the retention trial was conducted to complete the experiment.Results: The 10mg/kg and higher concentrations had greater step-through latency on day 2 compared with the scopolamine, control, and 5mg/kg concentration of test compound groups.Conclusion: Concentrations of 10mg/kg and higher of this particular aminopyrimidine derivative, 4-(4-trifluromethylphenyl)-2-aminopyrimidine, restore memory function to mice with memory deficits caused by scopolamine. Further studies are needed to find the mechanism of action of the novel compound.

کلیدواژه ها:

Aminopyrimidine ، Scopolamine-induced amnesia ، Learning and memory ، Passive avoidance