The antioxidant effect of aqueous extract of Artemisia annua on mitochondrial toxicity of kidney in type 2 diabetic male mice induced by high fat diet and Streptozotocin (STZ)

Type 2 diabetes is a worldwide metabolic disorder that is associated with an increased prevalence of obesity, an unhealthy lifestyle, and an aging process. As well as the general process of fatty acid metabolism in the liver, skeletal muscle and adipose tissue. In this study, Artemisia annua, which is found in south-east Asia and northern parts of Iran, was used to evaluate its effect on oxidative stress in kidney tissue in male mice. Oxidative stress is the result of the release of reactive oxygen species (ROS). Materials and methods: Mice were fed a high-fat diet for four weeks and then randomly divided into five groups of five. 1-Vehicle-Treated group (healthy group): In this group healthy animals received half a ml of Normal saline for 4 weeks. 2-Diabetic Vehicle-Treated Group: Animals in this group receive Normal saline as a solvent of the extract 7 days after confirmation of induction of type 2 diabetes for 4 weeks. Extract-Treated Diabetic Group: Animals in this group received intraperitoneal 100mg / kg aqueous extract of Artemisia annua daily for 4 weeks after confirmation of induction of type 2 diabetes 4- Extract-Treated Diabetic Group: In this group of animals, after confirmation of induction of type 2 diabetes for 4 weeks daily, the aqueous extract of Artemisia annua given 200 mg / kg according to its effective constituents. Receive intraperitoneally. 5- Extract-Treated Diabetic Group: In this group, animals receive 400mg / kg of aqueous extract of Artemisia annua daily for 4 weeks after confirmation of induction of type 2 diabetes. Oxidative stress parameters including the measurement of glutathione content, lipid peroxidation, and ROS in the kidney were investigated Results:Administration of Artemisia annua aqueous extract especially 400 mg / kg, resulted in a significant decrease in ROS production, followed by a decrease in lipid peroxidation and a reduction in glutathione reduction, which can inhibit diabet type 2 toxicity to be Conclusion:Our study showed that oxidative degradation plays an important role in a-induced liver toxicity, which is significantly inhibited by Artemisia annua aqueous extract.

کلیدواژه ها:

Oxidative stress ، kidney toxicity ، Artemisia annua ، diabetic type 2 ، mitochondrial toxicity ، STZ