Interaction between Tramadol and Methadone in kindling model of seizure and oxidative stress conditions in mice brain

Background: There are paradoxical information about role of tramadol in seizures, we aimed this study to assay role of opioid receptor in tramadol seizureMaterials and Methods: Tramadol (50 mg/mL) was infused at the constant rate of 0.5 mL/minute to mice. Infusion was cut if forelimb clonus followed by full clonus of the body. Dose of Tramadol (mg/kg mouse weight) to induce colonic seizures was as index of seizure threshold. Animals were divided into 4 groups of 6. Control group, tramadol (T) ,(Meth+T) and (Nal+T) group , 24 hours following injections, the animals were scarified .The brain tissues was assayed for malondialdehyde (MDA) ,glutathione (GSH) , Reactive Oxygen(ROS).Results:The minimal dose of Tramadol to induce colonic seizures was 140 mg/kg P<0.05 and Naloxone could have increased the threshold to 175 mg/kg , P<0,05,combination of tramadol with methadone decreased the threshold significantly. Also combination of Naloxone with tramadol improved oxidative stress but methadone before tramadol has not increased oxidative stress significantlyDiscussion: The μ opioid receptor cane be involved in tramadol seizure and naloxone can be used for preventing seizure by tramadol in emergency conditions but much more precise clinical trials are necessary.

کلیدواژه ها:

Tramadol ، Methadone ، Naloxone ، seizure ، oxidative stress ، in vivo