Evaluation of the expression level of ANRIL Long none coding RNA in the stool samples of patients with colorectal cancer, polyp adenoma and healthy individuals

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 365

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شناسه ملی سند علمی:

CIGS15_642

تاریخ نمایه سازی: 13 بهمن 1398

چکیده مقاله:

Introduction: A large number of lncRNAs have been shown to be involved in on cancer initiation, development and suppression. Colorectal cancer mostly detected at advanced stages, so early detection of the malignancy is important, and the use of noninvasive methods is preferable. In this study we evaluation of the expression level of ANRIL Lnc RNA, a 3.8 kb-long non-coding RNA expressed in the opposite direction from INK4A-ARF-INK4B , in the stool samples of patients with colorectal cancer, polyp adenoma and healthy individuals to find a non-invasive bio-marker.Materials and Methods: In this study, 20 stool samples from colon cancer patients, 20 stool samples from patients with adenoma polyp and 20 stool samples from healthy people were collected. Lnc RNA ANRIL expression was evaluated using Real Time PCR method. Fold change of gene expression was evaluated by (2-AAct) method.Results: up regulation of mRNA expression was found in ANRIL level in stool samples of CRC patients comparing to normal and polyp groups. These changes were linked with patient’s TNM stage, tumor differentiation and location (p˂0.05).Conclusion: The results of this study indicate that evaluation of ANRIL LncRNA expression in the stool sample can be used to as a non invasive biomarker for early detection in colorectal cancer.

نویسندگان

Shadi Sadri

Department of cellular and molecular, North Tehran Branch, Islamic Azad University, Tehran, Iran

Mitra Salehi

Department of Microbiology, North Tehran Branch, Islamic Azad University, Tehran, Iran

Ehsan Nazemolhosseini Mojarad

Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran