Evaluation of silver nanoparticles effects on Bap gene expression for biofilm formation in isolates of antibiotic-resistant Acientobacter Bumanni by real time PCR method
سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 324
نسخه کامل این مقاله ارائه نشده است و در دسترس نمی باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
CIGS15_602
تاریخ نمایه سازی: 13 بهمن 1398
چکیده مقاله:
Background and aim: Acinetobacter bomanni is one of the most common opportunistic pathogen in hospital that is resistant to many antibiotics due to the production of biofilm. This study aimed to evaluate an anti-biofilm activity of silver nanoparticles (AgNPs) on clinical antibiotic resistant Acientobacter bumanni.Materials and methods: In this experimental study, Acientobacter bumanni were isolates from 100 clinical samples. After identification of Acientobacter bummani strains and determination of antibiotic resistant profiles, biofilm producer isolates were determined using PCR method. The Minimum inhibitory concentration (MIC) of strains against AgNPs was determined. After 24 hours exposure of strains with subMIC concentration of AgNPs, RNA extraction and cDNA synthesis was performed. Finally, evaluation of Bap gene expression was measured using real time PCR method. Results: out of 100 clinical isolates, 12 isolates were belonged to Acientobacter bummani and all of strains were resistant to antibiotics except colistin. PCR results show that 12 isolates have Bap gene and they were biofilm positive. Real Time PCR results show that after treatment of isolates with subMIC concentration of AgNPs, all of strains had a significant reduction in Bap gene expression (P<0.05).Conclusion: According to anti-biofilm effects of AgNPs, it seems that AgNPs can be used as drug candidate in pharmaceutical industries.
کلیدواژه ها:
نویسندگان
Tohid Piri Gharaghie
Department of Biology, East Tehran Branch, Islamic Azad University, Tehran, Iran
Seyed Ataollah Sadat Shandiz
Assistant Professor, Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran