Biosynthesis and evaluation of anti-cancer effects of nanoliposome system containing essential oil of Ducrosia anethifolia (DC.) Boiss

Introduction. The plant Ducrosia anethifolia (DC.) Boiss, is native to Iran and its essential oil has cytotoxic effects. However herbal extracts have limited application due to their instability and being prone to oxidation. Nanoliposomes are spherical lipid bilayers which can encapsulate various active compounds including antibodies, proteins and aptamers and can facilitate medication delivery due to their resemblance to the cell membrane. The goal of this study was to develop nanoliposomal carriers of Ducrosia essential oil for use in biodrug delivery systems. Methods. The Ducrosia anethifolia (DC.) Boiss essential oil was first extracted and then gas chromatography and mass spectrometry were used to identify the extracted compounds. Thin-film hydration method was then used to create small liposomes loaded with the extract. The lipid phasewas prepared with L-alpha phosphatidylethanolamine dioleoyl, cholesteryl hemisuccinate and the essential oil. Finally, the cell toxicity of liposomal and free essential oil was compared on MCF7 cell line. Results. The employed methodology produced nanoliposomes measuring 269.8 nm in size with 100% frequency and polydisparity index of 0.507. Cell toxicity assay showed 60% increase in cancer cell death with liposomal essential oil. Conclusion. In this study, nanoliposomes containing Ducrosia anethifolia (DC.) Boiss essential oil were produced. Cell toxicity assay showed increased cancer cell death of liposomal essential oil compared to the free essential oil.

کلیدواژه ها:

Nanoliposomes ، Drug delivery ، Essential oil ، Ducrosia anethifolia (DC.) Boiss and MCF7.