Investigation of miR-210 expression in response to a VEGFB antagonist peptide in breast cancer mice

Breast cancer is the most common cancer in women worldwide. MicroRNAs, small non-coding RNAs, are pivotal regulators of cancer metastasis and progression through angiogenesis inhibition. MiR-210 is an important gene regulator induced under hypoxia conditions and acts as an oncogenic or tumor suppressor miRNA in different cancer types. VEGF signaling pathway plays a serious role in cell proliferation and angiogenesis. MiR210 regulate VEGF and VEGFR (angiogenesis key factors) expression and can be a main regulator of cancer progression. In this study we evaluated miR-210 expression level in Balb/c mice having 4T1 cell-line and treated with a VEGFB antagonist peptide. Breast samples were obtained from treated and untreated mice as case and control subjects, respectively. We extracted microRNAs by High Pure miRNA Isolation Kit (roche), after poly adenylation process cDNA was synthesized using anchored Oligo-dT. RT-PCR and Real-Time PCR. were performed in order to investigation of miR-210 expression level. miR-210 and U6 (as reference gene) specific primers were used. Our results showed a significant difference of miR-210 expression level between the case and control subjects. The results show that miR-210 may be a useful target for tumor metastasis and progression suppression strategies.

کلیدواژه ها:

miR-210 ، Breast Cancer ، Angiogenesis ، VEGFB