miR-142-3p Expression in Different Breast Cancer Cell Lines

Introduction: Breast cancer is the most common malignancy diagnosed in women, and the incidence of breast cancer is increasing every year. MicroRNAs are 22–25 nucleotide RNA segments that engage in post-transcriptional regulation by targeting messenger RNA sequences. These endogenous small non-coding RNAs play significant roles in tumorigenesis and tumor progression. miR-142-3p expression is dysregulated in several breast cancer subtypes. One of the aims of breast cancer treatment is to restore the expression of tumor suppressor miRNAs to normal levels in breast cancer cells by miRNA replacement therapy. Expression of Mir 142-3p decreases in breast cancer. This study aimed to evaluate the expression of Mir 142-3p in different breast cancer cell lines.Methods: Four main cell-lines including Mcf-7, MDA-MB231, MDA-468 and SkBR3 were chosen. After culture, their RNA was extracted and cDNA were performed. The micro RNA expression was determined using Real-Time PCR.Results: Mir 142-3p expression in MDA-MB-231 cell line was the highest among the others at the vicinity of 0.014. Whereas, its expression was approximately the same in cell lines MCF-7 and SKBR3 with around 0.007. The least expression levels belonged to cell line MDA-MB-468 which was roughly reached 0.004.Conclusion: In this study, we found that expression of Mir-142-3p in the MDA-MB468 cell line is less than the MDA-MB 231 and MCF-7 cell lines.

کلیدواژه ها:

miR-142-3p ، Breast Cancer ، Mcf-7 ، MDA-MB231 ، MDA-468 and SkBR3