The association of Tumor Necrosis Factor β gene polymorphism in active and inactive inflammatory bowel diseases

Background: Inflammatory bowel disease (IBD) includes two basic categories ulcerative colitis (UC) and Crohn s disease (CD) that the etiology of which remains unclear. Tumor necrosis factor Beta (TNFβ) promoter polymorphisms are a good candidate for susceptibility to IBD as there is a significant relationship between them. The main aim of this study was to assess TNFβ gene polymorphisms with IBD susceptibility at positions +252 in Iranian patients. Materials and Methods: In this case-control study, were studied 103 patients with IBD (85 ulcerative colitis, 18 Crohn s disease) and 100 healthy controls were studied. PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) was used for determining of genotyping. In following, allele frequency and genotype distribution of polymorphism A: G in TNFβ gene between the case and control groups were typed. To confirm the results of genotyping, 10% of the PCR products were sequenced using the ABI PRISM 3130xL Genetic Analyzer (Applied Biosystems®, Invitrogen Life Technologies, Carlsbad, CA, USA).Results: The frequency of genotype AA, AG and GG among Active patients was 37.7%, 46.4% and 15.9% and in Inactive patients was 65.6%, 21.9% and 12.5%, respectively(P=0.036). Also, allele frequency A+252 of TNFβ gene in IBD patients was high, while there is no statistical significant(P=0.375). Conclusion: There was significant correlation between TNFβ gene polymorphisms and susceptibility to IBD (Active and Inactive) at position +252. Our results showed that TNFβ gene polymorphisms can be considered as a potential prognostic marker cause of IBD in Iranian population.

کلیدواژه ها:

tumor necrosis factor beta ، polymorphism ، inflammatory bowel disease ، Iranian ، Crohn’s disease ، ulcerative colitis.