Study of Relationship between Gastric Cancer and EGFR Gene Mutations

Background: Gastric cancer is an aggressive disease that continues to have a daunting impact on global health. Despite an overall decline in incidence over the last several decades, gastric cancer remains the most common type of cancer and is the second leading cause of cancer-related death worldwide. The abnormal EGFR gene is involved in certain types of cancers, including gastric, prostate, cervical, ovarian, head and neck. EGFR belongs to tyrosine kinase family in respond to a Ligand binding, is activated through dimerization and autophosphorylation in tyrosine kinase domain. The purpose of this study was to investigate the relationship between EGFR gene mutations and gastric cancer.Methods: The samples were collected from 100 patients susceptible to gastric cancer as target group. DNA was extracted using VIOGENE kit. The mutation points detected by Gap-PCR and ARMs PCR then the results confirmed by Sanger Sequencing Results: The samples were collected from 100 patients susceptible to gastric cancer as target group. DNA was extracted using VIOGENE kit. The mutation points detected by Gap-PCR then the results confirmed by Sanger Sequencing Conclusion: The samples were collected from 100 patients susceptible to gastric cancer as target group. DNA was extracted using VIOGENE kit. The mutation points detected by Gap-PCR then the results confirmed by Sanger Sequencing

کلیدواژه ها:

Gastric canser ، EGFR ، Gap PCR ، ARMs PCR ، Sanger Sequencing.