Effect of DNA methylation and expression of CDK5 gene in patients with Oral Squamous Cell Carcinoma

Back ground: OSCC or oral squamous cell carcinoma is the sixth common type of cancer in the world and is the common form of malignancy of head & neck cancers. CDK5 has emerged an essential kinase in CNS and includes the process of neuronal maturation, migration, axon plasticity, cytoskeleton organization, endocytosis, exocytosis and apoptosis. Recently, CDK5 has been concerned in the development of a difference of cancers Materials and Methods: Samples are including 50 paraffin blokes of oral tissue case، and 50 biopsy of oral tissue for DNA methylation. The analysis of promoter hyper-methylation of CDK5 gene has done by MSP-PCR technique. Furthermore, the expression level of CDK5 gene assessed in 15 patients and 15 controls by real time PCR.Results: Promoter methylation of CDK5 gene was statistically different in OSCC patient in comparison to control. (OR=3.5, 95% CI=1.15-2.38, Pvalue=0.021). However, gene expression level of CDK5 gene was different in patient and healthy control (Mean SD, 1.55 5.99 respectively, Pvalue=0.001). Conclusion: This is a credible evidence of CDK5 gene methylation and expression in patient s with OSCC. This first hand attempt of epigenetic changes of the CDK5 gene in pathogenesis of OSCC.

کلیدواژه ها:

CDK5 ، OSCC ، MSP ، Methylation ، Expression.