Evaluation of cytotoxicity effects of synthetic compound imidazopyrimidin-3-amines derivatives as anticancer drugs on breast cancer line (MCF-7) by using MTT assay

Cancer, the second leading cause of death worldwide, is a heterogeneous disease which leads to uncontrolled cell growth. More than 100 types of cancer are known, and due to the progression of cancer, drug resistance, and side effects of current medications, researchers are looking for new synthetic drugs with fewer side effects to treat cancer. In this study, a series of synthetic drugs with anticancer activity previously prepared were tested. Since breast cancer is the most commonly reported cancers and is the leading cause of cancer death among women, the aim of current study was to identify and evaluate the cytotoxic activity of the synthetic drug of imidazopyrimidin-3- amines derivatives as anticancer agents and its effect on apoptosis in the breast cancer cell line, MCF-7, which ultimately reduces the growth of these cell lines and can contribute to cancer treatment. So far, the effects of 12 samples of imidazopyrimidin-3- amines derivatives in different concentrations of 50µm to 1000µm with a three-time repeat count on the cells of the breast cancer cell line have been investigated and Etoposide as positive control and DMSO as negative control have been used to determine the accuracy of the work. MTT assay was used for assay to measure the toxicity of the drugs. Our results showed that in MCF-7 cells, among the 12 available samples, IC50 were found in samples B9, B4, B2, B10, B6 at concentrations of 200µm, 300µm, 400µm, 500µm, 600µm.

کلیدواژه ها:

Apoptosis ، Cancer ، Cytotoxicity ، Drug