Immunomodulatory effects of active compounds extracted from secondary metabolite of Streptomyces Calvus on peripheral blood mononuclear cells (PBMCs)

Introduction: Today, microorganisms are considered as potential natural products for developing a novel pharmaceutical agent, and the use of natural compounds have less side effects. In this study, the immunomodulatory effects of active compounds of secondary metabolites of Streptomyces Calvus (S. calvus) evaluated on a gene expression of pro-inflammatory cytokines in human peripheral blood mononuclear cells (PBMCs).Methods: S. calvus was inoculated in Mueller Hinton Broth and secondary metabolites were extracted, then active compounds were isolated with HPLC technique. PBMCs were isolated from peripheral blood of healthy donor and were treated with active compounds. The cell proliferation was assessed by MTT assay and quantitative RT-PCR assays to determine mRNA expressions of TGF-β and INFɣ. Results: The secondary metabolite of S. calvus isolated into 7 different fractions via the HPLC. MTT assay results showed the 5th and 7th fractions have dose dependent immuno-activatory effect on PBMC. Also we didn’t see significant immuno-activatory effect in the 1st, 2nd, 3rd, 4th, and 6th fractions compared to untreated control. The 7th fraction could stimulate both TGF-β and INFɣ expression. However, the 5th fraction didn’t show any significant effect on TGF-β and INFɣ expression.Conclusion: This in vitro study showed that different active compounds of secondary metabolites of S. calvus can successfully stimulate human PBMCs. Therefore, these metabolites have the potential to serve as a robust immuno-modulator.

کلیدواژه ها:

Streptomyces calvus ، Active compounds ، Peripheral blood mononuclear cells ، INFɣ ، TGFβ