The study of the long non-coding GNAS-AS1 and BLACAT1 RNA expression in breast cancer tumor samples compared to the normal sample at the same time

Breast cancer is the most common cancer in women, and due to high rates of invasion and metastasis in these patients, the first cause of death is in women aged 40-44 years. The latest biomarkers and prognostic factors in a variety of cancers, including breast cancer, can be found in the form of Long Non-coding RNAs with a variety of oncogenic function or tumorigenic function (TSG), a marked change in the expression of this cancer showing normal breast tissue. The aim of this study was to simultaneously investigate changes in the expression of GNAS-AS1 and BLACAT1 non-coding gene expression in tumor tissue of breast cancer.Materials and Methods: MCF7 and MCF10A cancer cell lines were used to optimize the experiments. Then with the method qRT-PCR the expression of two non-coding RNA genes, GNAS-AS1 and BLACAT1, was investigated in 45 pairs of tumor and adjacent tumor fresh samples.Conclusion: According to the results of the study, 90 tumor samples of breast cancer were compared to the normal tumor margin of the same person, Non-coding RNAs of GNAS-AS1 and BLACAT1 have a pronounced increase in tumor tissue expression relative to the tumor margin (P = 0.0453 GNAS-AS1) (P = 0.0443 BLACAT1).Discussion: Considering the review of previous articles and micro array studies in human specimens, the existence of a possible hypothesis about the oncogenesis of two selected genes in the present study has been found Based on real-time PCR results and comparison of data, GNAS-AS1 and BLACAT1 genes increased significantly in the cancer cell line compared to the normal cell line and in the cancerous tissue compared to normal tissue which resulted in the probability of pro-oncogenesis of these genes.