improving gene transfer into myeloma cell by using microfluidic device

The potential use of gene- modified cell therapy in hematologic malignancies is often limited by complications related to effectively engineering and manufacturing cells by transfer of DNA and RNA, across the cell membrane with conventional delivery systems is challenge specifically for suspended blood cells. Herein, we compared two different approaches for gene transfer into myeloma cells : nucleofection as a 2D gene delivery and microfluidic device as a 3D gene transfer technology. Infact , we developed and used microfluidic chip for cell membrane poration that permits delivery of DNA into Multiple myeloma cell line. This device permeabilize cell membrane due to the Dean flow drag force, which tends to rotate and mix The cell, and the centrifugal force.. We achieved high transfection efficiency (37%% GFP) in myeloma cells with high cell viability (by trepan blue ) 24 hrs after microfluidic processing compared to nucleofection that is toxic and rate of dead cells is very high. We also demonstrate processing speeds of greater than 3.0 × 106 cells s−1 at volumes ranging from 0.5 to 1.5 mil liliters. The significant differences in outcomes from the two techniques underscores the importance of understanding the impact of intracellular delivery techniques on cell function for research and clinical applications .Altogether, these results highlight the use of microfluidic device as a rapid and gentle delivery method with promising potential to engineer primary human cells for research and clinical applications.

کلیدواژه ها:

gene delivery ، nucleofection ، microfluidic device ،