Nanoparticles synthesized by Stentrophomonas sp induced cell cycle changes in esophageal cancer cells

Introduction: Esophageal cancer is among the top ten leading causes of cancer-related death globally. Eastern to Central Asia are high incidence regions for this malignancy, including our country Iran. To develop more effective chemical agents against cancer, a lot of research has been currently focused on nanoparticles. Among different methods for synthesis of nanoparticles, use of microorganisms is a nontoxic and environmentally friendly approach. Herein, we evaluated effects of biosynthetic zinc and copper oxide nanoparticles (ZnNPs and CuONPs) on esophageal cancer cells by propidium iodide (PI) and flow cytometry.Methods: ZnONPs and CuNPs were synthesized by a psychrotrophic bacterium, Stentrophomonas sp. Then, esophageal cancer cells, KYSE30 cell line, were treated with 12.5 µg/ml ZnNPs and CuONPs for 24 h, while untreated cells were considered as control. Finally, cells were collected, stained with PI and analyzed by flow cytometry.Results and conclusion: Analysis of the cell cycle indicated that 55.7% and 34.3% of control cells were detected in G1 and G2/M phases, respectively. However, treatment with ZnNPs induced G2/M arrest, as 29.7% and 54.3 % of cells were detected in G1 and G2/M phases, respectively. More interestingly, CuONPs induced cell death as well as G2/M arrest, as 12%, 28.8% and 52.7% of cells were in sunb G1, G1 and G2/M phases, respectively. Accordingly, CuONPs induce more toxic effects on esophageal cancer cells, and therefore, it would serve as a good candidate for future anticancer studies.

کلیدواژه ها:

Biosynthetic nanoparticle ، Esophageal cancer ، Cell cycle analysis