The effects of Novel Bioactive Actinobacterial Metabolite in Wound Healing Process

Introduction: Previous studies have shown that Rare-Actinomycete Extract UTMC 522 has the potential for wound healing, so in this study, the capabilities of this extract are further explored as an effective factor in healing wounds.Methods: In this study, the sample prepared using a Eucerin ointment base with 1-20% AC.EX. 522 added. The circular wounds were created behind the neck of the Wistar rats and the ointment was applied to the position every day. The negative control group was treated with vehicle and positive control group was treated with phenytoin 1% ointment. The AC.EX. 522 (10 µl) was dissolved in 10 µl of DMSO and added to the cells culture medium (1–20 µg /ml). Proangiogenic activities were screened against human umbilical vein endothelial cells (HUVECs) by in vitro proliferation, tube formation, and migration assay, then planimetric, histological parameters and the expression of procollagen-I mRNA also VEGF analyzed.Results: AC.EX. 522 substantially influenced wound healing. The cream containing the highest dose of Extract (10%) significantly decreased wound size during the study, and histological evaluation showed Unique effects in the wound-healing process. Procollagen-I mRNA levels were significantly increased in the 10% AC.EX. 522 group compared with the vehicle and the phenytoin group control on postoperative day 7. VEGF levels were significantly increased in 10% AC.EX. 522 compared with positive and negative controls. AC.EX. 522 (5µg/ml) enhanced cell proliferation. It has increased the growth of cells 52% greater than control (cells + DMSO) and 20% greater than (cells + VEGF). AC.EX. 522 significantly increased the tubulogenesis compared with controls (P <0.001). AC.EX. 522 (5 µg /ml) promoted migration significantly (P < 0.001).Conclusions: AC.EX. 522 at a concentration of 10% accelerates the wound-healing process. In vitro and in vivo analyses, proves that AC.EX. 522 has a high capability for wound healing, so further studies are recommended to isolate the bioactive substance from this extract.

کلیدواژه ها:

Actinomycetes ، Wound healing ، Angiogenesis ، VEGF