Evaluation of Klebsiella Pneumoniae Carbapenemase-Producing Isolated from Wound Infectious in Isfahan, Iran

Background: Klebsiella pneumoniae is one of the most important opportunistic enteric bacteria and is a major cause of pneumonia and wound infectious. Potential of Klebsiella pneumoniae to produce K. pneumoniae Carbapenemase (KPC) is an important to hydrolyze carbapenems and other beta-lactam antibiotics. Carbanemase-producing bacteria can be characterized by Modified Hodge Test (MHT) and use of boronic acid as a KPC inhibitor. So we decided to identify KPC in K. pneumoniae isolated from bedsore wound infectious hospitalized in ICU of Isfahan.Methods: In this cross sectional study, 60 K. pneumoniae strains were isolated from bed sore wound infections of patients hospitalized in Isfahan. Biochemical tests were selected for K. pneumoniae characterization. Carbapenem-resistant was analyzed by disc diffusion method according to CLSI 2011 guideline. MHT and Boronic acid method was used for detection of Klebsiella pneumoniae carbanemase-producing which were examined by PCR method.Results: 53% of 60 isolates were resistant to carbapenem according to CLSI breakpoints and 27% were susceptible (P<0.05). MHT was positive in all of carbapenem-resistant isolates. According to results there were no synergism effect between boronic acid and meropenem and MHT has not enough specificity for detection of KPC and also 34% of isolates carried blaKPC gene.Conclusion: According to the results of this study, MHT is an important screening for detection of KPC in producing K. pneumoniae specifically in the patient with bed sore wound infections.

کلیدواژه ها:

Klebsiella Pneumoniae ، wound infectious ، carbapenems