The evaluation of the 2-deoxy-D-glucose effect on hippocampal levels of interleukins (IL-10 and IL-1β) in hippocampus in model of temporal lobe epilepsy in the male rat

Background and Aim : Epilepsy is a recurrent brain disorder. This condition has arisen from abnormal the excessive electrical activity of neurons in different parts of the brain. Temporal lobe epilepsy is one of the most common and drug-resistant type of epilepsy. ketogenic diet is a common treatment for controlling seizures in drug-resistant epileptic children and adult with refractory epilepsy. 2-DG is an analogue of glucose which can enter the cell through glucose transporters due to structural similarity with glucose. By inhibition of glycolysis, 2-DG diverts the energy pathway to the pentose phosphate shunt and mimics the ketogenic effects in the body. Epilepsy is always accompanied by inflammation. On the other hand, the increase of inflammatory cytokines in the brain can lead to the development of seizure. In some studies, the protective effects of 2-deoxy-D-glucose (2-DG) has been detected. in some neurodegenerative diseases such as Parkinson s, Alzheimer s. In this study, we try to measure the effects of 2DG on hippocampal levels of interleukins (IL-10 and IL-1β) in an experimental model of temporal lobe epilepsy in the rat.Methods : 50 male Wistar rats were randomly divided into 4 groups as follows: 1. Control/ vehicle group (2-DG solvent): n=10 2. 2-DG group (intraperitoneal injection of 125mg/kg 2-DG): n=10 3. KA group (injection of 0.8μg kainic acid in 1 μg normal saline into the left ventricle): n=10 4. 2-DG+KA (pretreatment injection of 125 mg/kg 2-DG half an hour before induction of epilepsy): n=10 Hippocampus of 25 epilepsy-induced rats was freshly removed and homogenized for detection of IL-1β and IL-10 concentration with ELISA test. The brain of 25 rats were used for immunohistochemistry detection of IL-10-secreting cells.Results : The level of IL-1β was significantly increased after epilepsy. However, in 2DG treated epileptic group the level of this inflammatory cytokine decreased, showing the anti-inflammatory properties of 2-DG. A significant increase in IL-10 concentration was also observed in KA+2-DG group vs KA group. The IHC results revealed positive IL-10 neuron in CA3, hilar and dentate gyrus regions of the hippocampusConclusion : 2-DG has an effective role in controlling inflammation in epilepsy by inhibiting IL-1β and producing IL-10 which is an anti-inflammatory cytokine.

کلیدواژه ها:

Temporal lobe epilepsy ، IL-1β ، IL-10 ، 2-deoxy-D-glucose (2-DG)