Effect of Testosterone and Voluntary Exercise on Angiogenesis in the sciatic nerve of type 1diabetic castrated male rats

Background and Aim : Diabetes mellitus (DM) is one of the metabolic disease that increasing prevalence worldwide. DM lead to endothelial dysfunction and reduces angiogenesis in nervous disease and peripheral coronary heart disease. Angiogenesis plays a critical role in the formation of new blood vessels in physiological and pathological conditions. This study was aimed to evaluate the effects of testosterone and voluntary exercise on density of vessels in the sciatic nerve of castrated diabetic rats.Methods : 48 male Wistar rats (weight: 250–300 g) were perfused with streptozotocin (50 mg/ kg) to cause type I diabetes. After 42 days of treatment with testosterone (2 mg/kg/day) and voluntary exercise, angiogenesis was determined in sciatic nerve by immunostaining for PECAM-1/CD31.Results : Our results in this study demonstrated that in Dia-Cas-T-E group significantly (p < 0.05) elevated the number of MVD in the sciatic tissue of diabetic rats compared to the diabetic group. In addition, in the Dia-T-E group, 6-week exercise training (p < 0.05) significantly increased the number of MVD in the sciatic tissue of diabetic rats compared to the diabetic group.Conclusion : Our findings suggest that testosterone therapy and exercise can promote angiogenesis in the sciatic nerve in rats.

کلیدواژه ها:

Angiogenesis ، Diabetes ، Sciatic nerve ، Testosterone ، Exercise