EFFECTS OF CANNABINOID RECEPTOR AGONIST AND ANTAGONIST ON PEAK AMPLITUDE OF SPIKES IN CEREBELLAR PURKINJE CELLS UNDER WHOLE CELL PATCH CLAMP RECORDING IN HARMALINE TOXICITY RATS

Background and Aim : : Cannabinoid receptors belong to the endocannabinoid system which are involved in different physiological procedures including memory, pain and appetite. In the Whole-cell patch-clamp recording, peak amplitudes of cerebellar purkinje cell spikes can be affected by changes in channels modulated by specific receptors or ligands so can be one of comparison factors in pharmacological research. The purpose of this study was to investigate the effects of cannabinoid receptor agonist and antagonists on peak amplitude of cerebellar purkinje cells under whole cell patch clamp recording. Methods : The peak amplitude in current clamp mode after IP injection of Harmaline, agonist WIN55, 212 2 and antagonists AM251 and SR171614a (Rimonobant) evaluated. The slices of cerebellar vermis prepared and under direct vision of purkinje cells and confirming the patch, recording done and in offline mode the peak amplitudes were caculated by Digidata 1440 and pclamp 10.1. The data were analysed by prism v6.Results : Comparison of the Peak amplitudes of action potentials in spontaneous activity of purkinje cells showed significant reduction by WIN administration in contrast to harmaline (H (4) =18.95; p=0.0008). Also some reduction seen in rimonabant group in comparison to harmaline (p< 0.05). Conclusion : According to the results, the changes in the agonist group are higher than in others but antagonist rimonabant showed improvement of that toward control group can be benefit effects on harmaline toxicity alongside of our previous behavioral study

کلیدواژه ها:

Cannabinoid ، harmaline toxicity ، WIN 212-2 ، Rimonabant