THE EFFECT OF DESCURAINIA SOPHIA OIL ON METHAMPHETAMINE INDUCED CELL CYTOTOXICITY AND CELL DEATH IN PC12

Background and Aim : Methamphetamine causes cytotoxicity and apoptosis in different cell lines. It seems that Descurainia Sophia oil, as an East Asian folk herbal drug, can suppress the methamphetamine-induced cell death. In this study, protective effects of Descurainia Sophia oil was followed up in methamphetamine-induced cell cytotoxicity and death in a neuron-like PC12 cell line.Methods : The viability, proliferation, and cytotoxicity of the cells, were assessed by Trypan blue, MTT test, and LDH assay, respectively. TUNEL test was performing to detection of DNA fragmentation and apoptosis. NO level was detected by Griese method.IL-1β, IL-6, INFγ, and TNFα pre-inflammatory cytokines were measured by Rat Kit V-Plex and finally caspase-3 activity was determined by spectrophotometry.Results : Results showed that Descurainia Sophia Oil has cell death-suppressing effects on nerve cells. It enhanced the cell viability and proliferation and also increased the cell cytotoxicity and cell death index in methamphetamine-treated PC12 cells. Also, it suppressed NO production, inflammatory cytokines production and secretion, mitochondrial membrane depolarization, and caspase-3 activity in a dose-dependent manner.Conclusion : We concluded that Descurainia Sophia oil suppresses the methamphetamine-induced cell death in PC12 cell due to reduction of NO production, cytokines synthesis, and inhibition of apoptosis cascade

کلیدواژه ها:

Descurainia Sophia ، methamphetamine ، PC12 cells