Computational and Experimental Study on the Interaction of Terbium(III) and Holmium(III) Complexes with Human Serum Albumin
سال انتشار: 1398
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 363
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شناسه ملی سند علمی:
ICCNRT02_021
تاریخ نمایه سازی: 30 آذر 1398
چکیده مقاله:
In this work, binding behavior of the terbium(III) and holmuim(III) complexes containing 1,10-phenanthroline (phen) ligand with human serum albumin (HSA) were studied by UV-vis, fluorescence and molecular docking examinations. The experimental data indicated that these lanthanide complexes have high binding affinity with HSA by effectively quenched the fluorescence of HSA via static mechanism. The binding parameters, the type of interaction, the value of resonance energy transfer and the binding distance between complexes and HSA were estimated from the analysis of fluorescence measurements and Förster theory. The thermodynamic parameters suggested that van der Waals interactions and hydrogen bonds play an important role in the binding mechanism. The results of molecular docking calculations assessed the microenvironment residues around the bound mentioned complexes and represent site 3 of HSA, located in subdomain IB, as the most probable binding site for these ligands. The computational results kept in good agreement with experimental data.
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نویسندگان
Zahra Aramesh-Boroujeni
Young Researchers and Elite Club, Najafabad Branch, Islamic Azad University, Najafabad, Iran
Meissam Noroozifar
Department of Chemistry, University of Sistan & Baluchestan, Zahedan, Iran