Intravitreal Injection of Zoledronic Acid in Rat Model of Choroidal Neovascularization

Purpose: Purpose: To determine the effects of intravitreal zoledronic acid (IVZA) on attenuation of laser-induced choroidal neovascularization (CNV) in rats. Methods: Methods: CNV was induced in 30 pigmented rats via laser burns. Rats were divided into two groups; one group received IVZA (8 μg / 2 μL) and another group received 2 μL normal saline. Neovascularization area was detected by lectin staining of choroidal-scleral flat mounts and quantified by using Image J software. Results: Results: Mean CNV area in the IVZA group (30016.02 ± 14182.82 μm2) was less than that of the controls (43935.49 ± 31554.54 μm2) with a borderline statistical significance (P = 0.059).Conclusion: Conclusions: IVZA with dose of 8 μg / 2 μL was effective in attenuation of CNV in rats. To maximize the antiangiogenic effect of IVZA, further investigation using the maximum safe dose of IVZA should be conducted.