New experimental skills for economical and faster slide preparation in morphological studies on phlebotomine sandflies

Background: Sandflies are main vectors in leishmaniasis in Iran and 97 countries in the world. Study on sandflies are doing for determination of fauna in the entire world.Objectives: Good slide preparation is many important in morphology and morphometric studies at phlebotomine sandflies. Routinely, 18×18mm cover glass and fixative are used for sample mounting.Materials and Methods: In the current study, small cover glass (4×4mm) and Pouri s medium as a fixative agent have been used in slide preparation. About 3000 slides of 20 species of sandflies have been prepared using this method.Results: Based on our experience in this study, the application of small slide glasses will be beneficial, economic and faster. In this experience immediately after mounting it could be placed in collection boxes especially in field conditions. This method can decrease the cost of study about 10% by reducing consumed materials such as slides, cover glasses, fixative, and boxes. 10 specimens could be mounted in each slide in average; it will decrease the number of collection boxes used for slide collection. This experience maybe applicable in other scientific studies inConclusion: New method in mounting of slides will be beneficial, economic and faster and it will decrease the cost of material in study (average 10% )

کلیدواژه ها:

Morphology ، Mounting ، Cost ، Economic ، Sandflies ، Beneficial ، Iran