Lucilia sericata Larval Secretion: In vitro and In vivo survey of its effects on Leishmania Major

Background:Leishmaniasis is a disease caused by an intracellular parasite of the Leishmania genus, which has been widespread around the world and Iran. In recent years, a tendency towards traditional and ancient remedies has been shaped; thanks to lack of response to treatment by pentavalent antimony compounds.Objectives: In this study, the effect of maggot secretions in vivo and in vitro on leishmania parasite and cutaneous leishmaniasis has been investigate Materials and Methods: 300 maggots of housefly named Lucilia sericata were provided in L3 stage and 500 maggots in sterile conditions. Maggot secretions were collected after 8hours in RPMI as a transfer medium. After determining the protein density, the impact ofsecretions in different densities on promastigotes and amastigotes of leishmania major was investigated in vitro using MTT color assay method in comparison to meglumine. In the next stage, the effects of secretions on the wounds of Balb/C mouse were examined.Results: Maggot secretions of L2 stage in six densities and maggot secretions of L3 stage in seven densities on L.major in vitro showed that an increase in the density of maggot secretions and confrontation time will rise the impacts of anti- Leishmaniasis. The results obtained from optical density and IC50 indicated that maggot secretions in comparison to the control drug had a significant effect. IC50 for maggot secretions of L2 was 10 μg/ ml and for L3 it was 24 μg/ml. The group of mice receiving maggot secretions of L2 stage with a density of 9.7 μg/ml and the group of mice receiving maggot secretions of L3 with a density of 12 μg/ml were compared mutually with Dunnett test, showing a significant effect.Conclusion: This study showed that maggot secretions had little toxicity impact on the macrophage. Also, the results of MTT test demonstrated that maggot secretions influenced the L.major parasite at all densities used, but in the in vitro conditions, it had a significant effect just in special densities and it is necessary to do more tests in order to evaluate these secretions in vivo.

کلیدواژه ها:

Lucilia Sericata ، Leishmaniasis ، maggot secretion ، MTT..