Evaluation of lefenuron, Ecofriendly and chitin synthesis inhibitor, on Culex pipiens

Background: West Nile virus (WNV) is a mosquito-borne virus that is mainly transmitted among birds by Culex pipiens . This species consists of pipiens and molestus biotypes, which together can form hybrids.Objectives: This study was done to determine the susceptibility status of Cx pipiens to Lefenuron as a insect growth regulator.Materials and Methods: The susceptibility status of field strains of Cx.pipiens were determined at Culicidae Insectary, Medical Entomology and Vector control, SPH,TUMS in 2017, using WHO technique. The laboratory-reared mosquito larvae, F1 of field-collected mosquitoes are exposed for 24 h to 48 h in water treated with the Lefenuron larvicide at various Concentrations, as 0.001-1000ppm, within its activity range, and mortality is recorded until the emergence of adults. LC50, LC90 values and the adult emergence inhibition (IE%) were obtained using Probit analysis with SPSS ver 16.0 software.Results: The LC50 and LC90 values for Lefenuron were measured as 0.624, 1.121 ppm respectively. More than 97% adult emergence inhibition was occurred in larvae recovered from treatments with test concentrations ranging from 10–1000 ppm.Conclusion: It is the first formal entomological research which defines LC50, LC90 values and the adult emergence inhibition of Lefenuron on Cx.pipiens in Iran. Future studies are recommended to identify Larvicidal and adult emergence inhibition effect of Lefenuron in semifield and field conditions.

کلیدواژه ها:

Culex pipiens ، Lefenuron ، Susceptibility ، Iran