Background: A diabetic foot ulcer is a devastating complication and is estimated to influence 15% of all diabetic individuals throughout their life. Unfortunately, 5–24% of foot ulcers will finally lead to limb amputation. There are multiple reports throughout the world about the use of larval therapy in the treatment of diabetic foot ulcers, but in Iran, this treatment is very little considered. So, the present study aimed to investigate the effect of larval therapy on healing of diabetic foot ulcers, removal of necrosis tissue, and prevention of limb amputation.Materials and Methods: The present study is applied research that was conducted in the Saghi clinic in Tehran city, Iran, on a patient with a diabetic ulcer on his right foot. The patient s physicians ordered amputation after routine treatment and failure to obtain the desired results. At the mentioned wound care center, the patient was treated by larval therapy. Larval therapy was performed in seven separate 48-hour sessions using sterile Lucilia sericata larvae. After each larval therapy session, bacterial contamination of the wound was sampled by sterile swab.Results: At different steps of larval therapy, necrotic tissue and bacterial contamination were observed to heal and disappear gradually. In the latter step, no necrotic tissue or bacterial contamination was observed and the wound was completely healed without surgery and the patient was discharged.Conclusion: The results of the present research demonstrated the efficacy of larval therapy in the removal of necrotic tissues and prevention of diabetic foot ulceration. Although many questions are still unanswered and more surveying are needed, it is recommended to use larval therapy as an effective suggested treatment for diabetic foot ulcers and further studies will be conducted in this area