The comparison of cytotoxic effects of essential oil of Nepeta crispa willd and Malathion insecticide on the growth of cultured vertebrate (L929) and invertebrate cells (Sf9)

سال انتشار: 1398
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 422

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شناسه ملی سند علمی:

DCME02_040

تاریخ نمایه سازی: 19 آذر 1398

چکیده مقاله:

Background: Resistance of insects to insecticides, entry of poisons into the ecosystem and food chain, extermination of non-target organisms, mutation, and pollution of soil and water had been critical problem in the recent years. The use of botanical compounds and essential oils can be considered as an appropriate tool to control pests and pathogen vectors.Objectives: pesticides are used as essential tools to control vector-borne diseases and agricultural pests and maintain quality and quantity crop production. Scientists attempt to use derived plant natural products due to environmental safety and low mammalian toxicity. Therefore, the cytotoxicity of malathion and Nepeta crispa essential oil against vertebrate L929 and invertebrate Sf9 cell lines were investigated.Materials and Methods: about 2×103 cells were placed into the wells of a 96-well plate experiments. Then appropriate concentrations of malathion and N. crispa essential oil added to the wells. The cells were allowed to grow for 3-5 days and estimated the cell numbers. Control cell wells contained only cells with DMSO. All treatments and controls repeated at least four replicates. The most effective concentration was found between the concentrations used. The data was analyzed using SPSS software, ANOVA (TOKEY statistical test).Results: The results showed that N. crispa essential oil had a negative effect on Sf9 cells and decreased its growth viability, while Nepeta crispa essential oil cytotoxity effect on the Sf9 cell line, at concentrations of 10 -7 -10 -3 μg-μL that was significant. There was no cytotoxic effect of N.crispa essential oil on L929 fibroblast cells and the viability of these cells increased growth following essential oil reatment.Conclusion: plant essential oil not only had no negative effects but also had boosting effects on the L929 cell viability. Nepeta crispa essential oil had negative effects on the Sf9 cell viability with the differences that derived plant natural products containing environmentally friendly and readily biodegradable derivatives, hydrolyzing rapidly in nature and nearly having no destructive effects on mammals and environment

نویسندگان

Amirhossein Zahir nia

Associate Professor, Department of Medical Entomology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

Mitra Boroomand

Ms of medical entomology, Department of Medical Entomology and Vector Control, School of medicine, Hamadan University of Medical Sciences, Hamadan, Iran

Hassan Nasirian

Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

Sara Soleimani-Asl

Department of Anatomy, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

Aref Saleh zadeh

Associate Professor, Department of Medical Entomology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

Dara Dastan

Medicinal Plants and Natural Products Research Center, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran