Study on cell growth and proliferation of nHA/GO/M composite

Nano Hydroxyapatite (nHA) [Ca10(PO4)6OH10] is a good selection for different applications because of its similarity of synthesis to mineral phase of the bone, excellent biocompatibility, ability to promote cellular functions andosteoconductivity.Nano Hydroxyapatite/ graphene Oxide/ doped with metal ions (nHA/GO/M) were prepared using the chemical precipitation method[1].Zinc (Zn) andMagnesium (Mg)were selected as metal ions (M=Mg, Zn).Synthesized samples were identified by the Fourier transform infared (FTIR), Field emission scanning electron microscope (FESEM), X-ray diffraction (XRD), methods. XRD pattern reveals that all reflection peaks of each sample still corresponded to the nHA phase, and that no other phase could be detected. FESEM results showed that nHA andnHA/GO/Zncomposites displayed rod-like and cluster shapes, respectively [2]. An MTT assay was executed to test the in vitro cytotoxicity of doped nHA composites aginst L929 cell lines. The results of the cytotoxicity assay revealed an acceptable limit for the cell growth and proliferation.