The Abortifacient Effect of Petroselinum Crispum Seed Extract and Its Correlation with ENAC gen Expression and Progesterone in Balb/c mice

سال انتشار: 1398
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 308

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شناسه ملی سند علمی:

COBGY15_104

تاریخ نمایه سازی: 11 آبان 1398

چکیده مقاله:

Background & Aim: The herb, Petroselinum crispum, is a perennial fragrant plant which cultivates widely in the Iran as eating vegetables. Petroselinum crispum is an Iranian medicinal plant which is used for various diseases. The leaves of the plant are taken in folk medicine by certain women of Iran for abortion induction. However, there is no scientific evidence to prove its side effects in pregnancy. The aim of this study was to assess the seed extract of the plant on placenta implantation and abortion, in Balb/c mice.Materials and Methods: The present experimental study was conducted on 30 adult female Balb/c mice. The animals were divided into 6 groups of 5 specimens, including non-pregnant control, pregnant control, and three sets of empirical receiving doses 5 mg/kg body weight extract of parsley seeds after mating and vagina platelets observation respectively. First group of treatment animals received 5 mg/kg body weight extract of parsley seeds in the first week, the second group received the extract in the second week and the third group received the extract in the third week. One group received 5 mg/kg body weight extract of parsley seeds for 21 days continually. At the end of the experiment, caesarean surgery was done and pregnancy factors including number of placenta, number of embryos, abortion rat, and progesterone concentration of serum were measured. The level gene expression of ENAC was also measured by real time PCR method to evaluate the mechanism. Uterus tissue were removed and fixed in 10 % neutral formalin and embedded in paraffin. 5-μm-thick sections were prepared and stained with hematoxylin and eosin by standard procedures. The Data were analyzed with one way ANOVA and Tukey’s post-hoc test and significant differences of data was considered at p = 0.05.Results: There was only a significant difference in progesterone level in case group that received extract continually for three weeks vs. pregnant control group (p<0.05). The abortifacient effect of the plant was supported by the changes in number of placenta and number of embryos in treatment group that received extract continually for three weeks vs. pregnant control group (p<0.05). The percentage of abortifacient increased in all treatment groups compared with pregnant control group. Meanwhile, the administration of 5mg/kg of the extract in all groups of gestation exhibited dramatic abortion. For evaluate the mechanism action of parsley, the level gene expression of ENAC was measured by real time PCR. There was no significant difference in studied groups regarding the level gene expression of ENAC. Histopathological analysis showed that some areas of placenta were necrosis and also decidua degeneration in all groups that treatment with parsley was seen. Since, parsley caused a drastic harm of the all pregnancy factors of mic, as shown in this study; therefore, taking the seed extract of the plant by pregnant women is not advisable.Conclusion: Considering parsley seed extract effect on hormone changes in pregnancy and abortifacient effect of parsley in experimental group, its consumption should be with cautious during pregnancy. These results showed that mechanism action of parsley seed extract is not done via change in gene expression of ENAC channels and these effects may be related to change in hypothalamus-hypophysis- gonad pathway.Further studies can also be conducted to determine the active constituent of parsley seed extract that gives abortifacient property to this plant.

نویسندگان

Hengameh Dashti Rahmat Abadi

in Cellular and Molecular Biology