The Carcinogenic Roles of MicroRNA-613andMicroRNA-223inthe Epithelial Ovarian Cancer:APilot Study

It is generally accepted that ovarian cancer is the leading cause of death among womenworldwideasa most deadly cancer in the female reproductive system, and is often diagnosedatdevelopedstages.MicroRNAsplay critical roles in the initiation and progression of cancer, metastasis and resistance to different therapies as biological molecules that regulate thegene expression.Studies have found that K-RASprotein of miR-31targets is significantly important in thecarcinogenesis of ovarian cancer.The present study also aimed to compare microRNA-613andmicroRNA-223levels insample patients with ovarian cancer.Method: In this study, 25samples of malignant ovarian cancer, 25 samples of benign ovarian cysts and 5 samples of healthy ovaries were collected and stored in -700C.RNA was extracted andcomplementary DNA (cDNA) was prepared by Cdna synthesis kit. The gene expression level of MicroRNA-613 and MicroRNA-223was evaluated by Real Time PCR method. Results were analysed by SPSS21 software and one way ANOVA statistical method.Results: According to an analysis ofobtained results of Real-time PCRtechniqueby a relative method in two groups of patients with ovarian cancer and benign and malignant tumors, and a healthy group, the relative expressionof miRNA-223in samples of malignant tumors, benign tumors and healthy ovaries was 1.94, 0.64, and 1.26respectively (p= 0.01).These results indicated thatthe relative expressionof miRNA-613insamples of malignant tumor, benigntumor and healthy ovaries was 0.65, 2.13, and 1.78, respectively. (p=0.05).Conclusion: We can concludethat miRNA-613andmiRNA-223have potential for introduction as research targets in biomarker discovery studies for early diagnosis of metastasis in patients with ovarian cancer.They can also be effective in anticipating the response to treatment, and play potential roles in developing therapeutic approaches and predict treatment outcomes in ovarian cancer patients.

کلیدواژه ها:

Ovarian Cancer ، miRNA-613andmiRNA-223.