Curcumin and Dendrosomal Nanocurcumin Effects on Androgen Receptor Gene Expression in Endometrial Tissue Culture of Women with Polycystic Ovary Syndrome

Background: Polycystic ovary syndrome (PCOS) is a common gynecological disorder, affecting 5–7% of women of reproduc-tive age, and characterized by oligo-/anovulation, polycystic ovaries morphology and elevated circulating concentrations of androgens and/or signs of hyperandrogenism. It is well estab-lished that the cellular actions of androgens are mediated via its binding to and activation of androgen receptor (AR). PCOS en-dometrium overexpresses AR. In fact, many of the consequenc-es of hyperandrogenism such as hirsutism, acne, hyperinsuline-mia as a result of insulin resistance, and implantation failure are due to the elevated expression of AR in endometrium of PCOS women. However, several studies have shown that curcumin (a phenolic curcuminoid derivative from Curcuma longa) has ben-eficial effects on PCOS induced rats. Our aim was to determine the effects of curcumin and dendrosomal nanocurcumin (DNC, nanoformulation of curcumin), in endometrial tissue culture of women with PCOS.Materials and Methods: Endometrial biopsy was taken from a PCOS woman. After stromal cell culture, MTT assay was exploited to measure their viability against curcumin, DNC, DMSO and nanocarrier (DMSO and nanocarrier as the SHAM groups) treatment. Cells were separately subjected to these sub-stances for 48 hrs. Afterward, RNA extraction, DNA synthesis and real-time PCR were applied to examine and analyze the mRNA expression level of the AR gene.Results: Our data revealed that curcumin treatment causes to a significant decrease in mRNA expression level of the AR gene in all treated cells compared to control group.Conclusion: This primary finding implies the dynamic role of curcumin in gene expression pattern of endometrial cells in women with PCOS; although we re investigating these treatments with more sample number to get more reliable results.

کلیدواژه ها:

Polycystic ovary syndrome ، Androgen receptor ، Hy-perandrogenism ، Curcumin ، Nanocurcumin