Investigation of Serum Extracellular Vesicles De-rived microRNAs in Endometriosis

Background: Recent studies offer a non-invasive method for detection of endometriosis based on extracellular vesicle miR-NAs as potential biomarkers. However no reliable biomarker has been introduced yet. Level expression of miRNAs change in progression of endometriosis.Materials and Methods: PubMed search using micro RNA and endometriosis in title/abstract or Mesh terms was used to find out previously reported candidates following by bioinfor-matics analysis based on mirbase, miRTarBase, enrichr, mir-namap. Twenty women with histologically proven severe endo-metriosis and 20 endometriosis-free were enrolled in this study to check existence of potential candidate miRNA in extracel-lular vesicle. All samples collect in morning.Results: Six miRNAs including miR-451a, miR-199a, miR-100, miR-148a, miR-154 and miR-23b based on literature re-view (114 article between 2007 and 2018) and bioinformatics analysis were selected and their expression level in extracted EVs between samples showed some differences.Conclusion: Golden standard method for diagnosis is laparos-copy that is an invasive and costly method. In addition, sam-pling of ovaries in endometrioma can decrease reserve oocytes and lead to subfertility or infertility. Hence use of a non-inva-sive method such as biomarker is suggested. Significant altera-tion in serum extracellular vesicles derived candidate microR-NAs can consider them as a potentially biomarker for detection of endometriosis.

کلیدواژه ها:

Endometriosis ، microRNA ، extracellular vesicle ، Biomarker ، Bioinformatics analysis