Effect of Cholestasis on Claudine-11 Gene Expres-sion in Testis of Male Wistar Rats

سال انتشار: 1398
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 264

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شناسه ملی سند علمی:

RROYAN20_383

تاریخ نمایه سازی: 29 مهر 1398

چکیده مقاله:

Background: Cholestasis is a liver disease that results in the destruction of testicular tissue. One of the most important fac-tors in maintaining the testicular tissue integrity is the blood-testis barrier (BTB). The Claudine-11 gene is expressed in sertoli cells and plays important role in the blood-testis barrier construction. The purpose of this study was to investigate the effect of cholestasis syndrome on BTB and the expression of Claudine 11 in male rats.Materials and Methods: : In this study, 12 male rats were di-vided into control and cholestasis groups. To induce cholestasis, the bile duct was closed during surgery. The testicular barrier permeability was evaluated after 3 weeks using Evans Blue s staining. Claudine 11 gene expression was analyzed using real time PCR method and the data were analyzed by one-way vari-ance using SPSS software.Results: The results of this study indicated that cholestasis syn-drome leads to a change in the permeability of BTB, so that the permeability of BTB increased almost more than 2 times in the cholestasis group compared to the control group. The results of gene expression analysis also showed that the relative ex-pression of Claudine 11 gene in testis tissue of cholestasis was significantly lower than healthy animals.Conclusion: Cholestasis is one of the diseases that can lead to fertility problems in men. The present study showed that chol-estasis can damage the testicular tissue by destroying the blood-testis barrier and altering the expression of genes involved in intercellular connections.

نویسندگان

E Rahmani

Department of Animal Bioligy, Faculty of Biological Sciences,Kharazmi University, Tehran, Iran

H Mohseni Kouchesfehani

Department of Animal Bioligy, Faculty of Biological Sciences,Kharazmi University, Tehran, Iran

H Jalali

Department of Animal Bioligy, Faculty of Biological Sciences,Kharazmi University, Tehran, Iran