Protective Effect of Vitamin E on Expression of P53 Gene and FSH/LH Levels in Infected Rats with Candida. Albicans

Background: Various microbial agents with different patho-logic mechanisms may affect sperm function by infecting the urogenital tract at different sites The aim of this study was to investigate the harmful effects of C. albicans on Expression of P53 gen and Equal amounts of total protein of P53, Serum levels of Follicle Stimulating Hormone (FSH) and luteinizing hormone (LH) Levels in infected Rats with C. albican.Materials and Methods: The treatment of 24 Adult rats was performed in four groups: control, infected with C. albicans, infected with C. albicans + Vitamin E, Vitamin E (100mg/kg/ day) for 5 weeks. The expression of P53 gene was analysed by real-time PCR and expression of p53 gene, also Equal amounts of total protein of P53 was analysed by Western Blot and Serum levels FSH and LH were determined with the enzyme-linked immunosorbent assay method.Results: In the present study no significant difference was ob-served in the serum level of FSH C. albicans-exposed rats. Con-versely, our results showed significantly decreased of LH and mRNA and protein expression during treatment of animals with C. albicans compared to the control group. While C. albicans +Vitamin E group showed a highly significant increase in LH and mRNA expression but no significant difference of protein expression compared to C. albicans group. Conclusion: Vitamin E could compensate the adverse effects of C. albicans on Serum level of LH, mRNA and protein expres-sion in adult rats.

کلیدواژه ها:

P53 ، Vitamin E ، FSH ، LH ، C. albicans