Increase Expression of IL-1b Transcriptin CulturedPBMCs

سال انتشار: 1398
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 283

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شناسه ملی سند علمی:

RROYAN20_331

تاریخ نمایه سازی: 29 مهر 1398

چکیده مقاله:

Background: There is strong evidence that cytokines and growth factors play an important role as local mediators of the actions of steroids on the endometrium to prepare it for implan-tation. In humans, PBMCs were reported to induce the produc-tion of several cytokines, inflammatory cytokines such as IL-1b which play a key role in primary immune responses. In this article, the impact of intrauterine administration of PBMCs on the expression of il1-b transcript and role il1-b in embryo im-plantation and pregnancy rate.Materials and Methods: Pregnant mice were randomly divid-ed into two groups, including embryo implantation dysfunction (EID) group; EID with PBMCs group. Mouse PBMCs were isolated from whole blood of the non-pregnant female mouse and then cultured 0, 24, or 48 hours in vitro. Uterine horns were excised to determine the number of pregnant mice and implan-tation sites on the day 7.5 postcoitum. mRNA expressions of interleukin-1b (IL-1b) in the cells were examined using the quantitative real-time polymerase chain reaction analysis (real-time PCR).Results: IL-1b transcript was expressed in mouse cultured-PB-MCs. PBMCs significantly increased IL-1b (P < 0.05) mRNA level in mouse PBMCs. This result showed that the expression of IL-1b mRNA in mouse PBMCs was transiently increased. Conclusion: Intrauterine administration of mouse PBMCs de-rived from unpregnant mice prior to embryo implant have a good influence on endometrial receptivity and embryonic im-plantation in EID mice.

کلیدواژه ها:

Embryo Implantation Dysfunction ، Peripheral Blood Mononuclear Cells ، Endometrial Receptivity ، il-1b ، Mouse

نویسندگان

D Rezaee

Department of Medical Biotechnology, School of Advanced Tech-nologies in Medicine, Shahid Beheshti university of Medical Sciences, Tehran, Iran

M Salehi

Department of Medical Biotechnology, School of Advanced Tech-nologies in Medicine, Shahid Beheshti university of Medical Sciences, Tehran, Iran

M Bandehpour

Department of Medical Biotechnology, School of Advanced Tech-nologies in Medicine, Shahid Beheshti university of Medical Sciences, Tehran, Iran

B Kazemi

Department of Medical Biotechnology, School of Advanced Tech-nologies in Medicine, Shahid Beheshti university of Medical Sciences, Tehran, Iran